БЕЗКЛІТИННІ БІОЛОГІЧНІ ЗАСОБИ: ФОКУС НА КОНДИЦІОНОВАНІ СЕРЕДОВИЩА МЕЗЕНХІМАЛЬНИХ СТОВБУРОВИХ КЛІТИН
DOI:
https://doi.org/10.32782/2226-2008-2023-4-15Ключові слова:
безклітинні біологічні засоби, кондиціоноване середовище, позаклітинні везикули, екзосоми, культиваційне середовищеАнотація
Огляд присвячено узагальненню сучасних відомостей про кондиціоновані середовища мезенхімальних стовбурових клітин (КС-МСК), як інноваційний безклітинний біологічний засіб. Термін «кондиціоноване середовище» належить до рідкої фази середовища клітинної культури, збагаченої секретомом культивованих клітин. Характеристики КС-МСК різняться залежно від власне джерела вихідних МСК (кістковий мозок, жирова тканина, плацента та ін.), газових умов культивування – нормоксія (O2 20,0–21,0 %) або гіпоксія (0,5–1,0–1,5–2,0 % О2), тривалості культивування (від 16–24–48–72 годин до 3–5 днів) та ін. Культуральне середовище в культурі in vitro являє собою мікрооточення в умовах in vivo та може визначати долю клітин і, таким чином, їхні паракринні властивості. Застосування КС-МС має переваги над власне МСК за рахунок відсутності імуногенності. Натепер у світі зареєстровано 14 клінічних досліджень щодо ефективності кондиціонованих середовищ.
Посилання
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