NEUROPROTECTIVE EFFECTS OF DEPROTEINIZED CALF BLOOD HAEMODIALYSATE IN CASE OF INTRANASALLY ADMINISTRATION UNDER CHRONIC CEREBRAL ISCHEMIA

Abstract

Statistical data on acute cerebrovascular accident rates in our country highlight the progressive increase in new stroke cases. Such an extremely unsatisfactory situation has arisen due to some reasons, one of which is the ineffective pharmacotherapy of strokes, which is a sequence of our incomplete understanding of chronic cerebral ischemia pathophysiological mechanisms. It seems interesting and expedient to clarify the efficacy of intranasal administration of deproteinized haemoderivative of young calves’ blood throughout the post-ischemic period from the point of view of the potential restoration of impaired mnestic functions. The aim of the work was to determine the efficacy of endonasal administration of deproteinized calf blood dialysate for restoring memory impairment in rats throughout the post-ischemic period dynamics. Materials and methods. A model of chronic brain ischemia was reproduced by carotid arteries bilateral isolation and ligation. Rats with chronic brain ischemia were intranasally administered deproteinized calf blood dialysate. The rats were observed for 7 days, during which the animals’ mnestic functions were examined in the forms of active avoidance conditioned reflex and a conditioned food reflex development. Results and their discussion. The data obtained indicate the formation of mnestic dysfunctions during the postischemic period, which are characterized by learning process decline as well as short– and long-term memory marked suppression under active avoidance conditioned reflex, which is combined with conditioned food reflex learning, preservation and extinction processes disturbances. The registered amnestic effects are persistent, noted 24 hrs after the carotid arteries occlusion and continued throughout the whole trial. Considering the mechanisms for achieving neuroprotective effect of deproteinized calf blood dialysate and its intranasal route of administration, the authors suppose it appropriate and pathogenetically justified to include deproteinized calf blood dialysate in a comprehensive scheme of pharmacological correction of cognitive disorders or their prevention under cerebrovascular pathology.