АНАЛІЗ ВПЛИВУ ВИСОКИХ ДОЗ 2-((4-АМІНО-5-(ТІОФЕН-2-ІЛМЕТИЛ)-4Н-1,2,4-ТРИАЗОЛ-3-ІЛ)ТІО) АЦЕТАТУ НА ТКАНИНУ МОЗКУ
DOI:
https://doi.org/10.32782/2226-2008-2025-1-15Ключові слова:
1,2,4-триазол, мозкова тканина, токсична діяАнотація
Стаття присвячена дослідженню тканин мозку у разі введення високих доз (1000 мг/кг і 5000 мг/кг) 2-((4-аміно-5-(тіофен-2- ілметил)-4Н-1,2,4-триазол-3-іл)тіо)ацетату перорально за допомогою зонду. Доза в 10 разів більша за ефективну дозу (100 мг/кг – щури) – 1000 мг/кг уже призводить до змін гістологічної структури тканин органів. Максимальна аргументована доза четвертого класу токсичності 5000 мг/кг також викликає серйозніші зміни у структурі тканин органів. Таким чином, наше дослідження дало докази зміненої гістологічної архітектури тканини мозку.
Посилання
Sameliuk Y, Kaplaushenko T, Al Zedan F. 1,2,4-triazole derivatives in medicine and pharmacy and application prospects. Journal of Faculty of Pharmacy of Ankara University. 2021; 45(3):598–614. https://doi.org/10.33483/jfpau.885888.
Shcherbyna R. Microwave-assisted synthesis of some new derivatives of 4-substituted-3-(morpholinomethyl)-4h-1,2,4-triazole-5-thioles. Journal of Faculty of Pharmacy of Ankara University. 2019; 43(3):220–229. https://doi.org/10.33483/jfpau.533166.
Dovbnia D, Frolova Y, Kaplaushenko A. А Study of hypoglycemic activity of acids and salts containing 1, 2, 4-triazole. Ceska a Slovenska Farmacie: Casopis Ceske Farmaceuticke Spolecnosti a Slovenske Farmaceuticke Spolecnosti. 2023; 72(3):113–124. PMID: 37648427.
Shcherbyna R. An investigation of the pharmacokinetics and potential metabolites of potassium 2-((4-amino-5-(morfolinometyl)-4H-1,2,4-triazol-3-yl)thio) acetate on rats. Journal of Faculty of Pharmacy of Ankara University. 2020; 44(2):233–241. https://doi.org/10.33483/jfpau.681611.
Shcherbyna R, Vashchyk Y. The research of 1,2,4-triazole derivatives hepatoprotective activity under tetracycline and infectious hepatitis. Journal of Faculty of Pharmacy of Ankara University. 2019; 43(2):135–146. https://doi.org/10.33483/jfpau.487173.
Gotsulya A. Synthesis and antiradical activity of alkyl derivatives of 5-(5-methyl-1H-pyrazol-3-yl)-4-phenyl-4H-1,2,4-triazole-3-thiol. Journal of Faculty of Pharmacy of Ankara University. 2020; 44(2):211–219. https://doi.org/10.33483/jfpau.616116.
Gotsulya A, Zaika Y, Brytanova T. Synthesis, properties and biological potential some condensed derivatives 1,2,4-triazole. Journal of Faculty of Pharmacy of Ankara University. 2022; 46(2):308–321. https://doi.org/10.33483/jfpau.971602.
Safonov A, Panasenko O. Synthesis, antimicrobial and antifungal activity of 3-(2-bromophenyl)-5-(alkylthio)-4-phenyl-4H-1,2,4-triazoles. Current issues of pharmaceutical and medical science and practice. 2022; 15(3):235–240. https://doi.org/10.14739/2409-2932.2022.3.264691.
Knysh Y, Panasenko O, Safonov A. Sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio)acetate, which shows actoprotective activity: patent 112619 Ukraine, 2016. [CrossRef]
Ghanem CI, Pérez MJ, Manautou JE, Mottino AD. Acetaminophen from liver to brain: New insights into drug pharmacological action and toxicity. Pharmacological research. 2016; 109:119–131. https://doi.org/10.1016/j.phrs.2016.02.020.
Jain S, Ahmed RS, Arora VK, Banerjee BD. Biochemical and histopathological studies to assess chronic toxicity of triazophos in blood, liver and brain tissue of rats. Pesticide biochemistry and physiology. 2011; 100(2):182–186. https://doi.org/10.1016/j.pestbp.2011.03.00.
Safonov AA, Panasenko OI, Al Khalaf NA. Study of the effect of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl) thio) acetate toxic doses on rats’ heart tissue. Current issues in pharmacy and medicine: science and practice. 2024; 17(2):150–153. https://doi.org/10.14739/2409-2932.2024.2.300859.
Safonov A, Demianenko D, Vashchyk Y, Larianovska Y, et al. Histological study of a corrective influence of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio) acetate on the state of rats liver under conditions of acute immobilization stress. Journal of Faculty of Pharmacy of Ankara University. 2022; 46(2):330–341. https://doi.org/10.33483/jfpau.1012893.
Safonov A, Demianenko D, Vashchyk Y, Lytkin D, Polonets O, Kosareva A, Morozova L. Study of the Stress-Protective Effect of Sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio)acetate. HUJPHARM. 2024; 44(2):143–52. https://doi.org/10.52794/hujpharm.1374871.
Ministry of Health of Ukraine. Guideline. Medicinal Products. Preclinical Safety Studies for Clinical Trials Involving Humans and the Registration of Medicinal Products (ST-N MOZU 42-6.0:2014). Kyiv: Ministry of Health of Ukraine. 2014. Available from: https://zakon.rada.gov.ua/rada/show/v0661282-14#Text.
Guide for the Care and Use of Laboratory Animals. Washington, D.C.: National Academies Press, 2011. https://doi.org/10.17226/12910.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of the European Union. 2010; 276:33–79 [CrossRef].
Xu L, Shao A, Zhao Y, Wang Z, Zhang C, Sun Y, Chou LL. Neurotoxicity of silver nanoparticles in rat brain after intragastric exposure. Journal of nanoscience and nanotechnology. 2015; 15(6):4215–4223. https://doi.org/10.1166/jnn.2015.9612.
Olayode OA, Daniyan MO, Olayiwola G. Biochemical, hematological and histopathological evaluation of the toxicity potential of the leaf extract of Stachytarpheta cayennensis in rats. Journal of traditional and complementary medicine. 2020; 10(6):544–554. doi: 10.1016/j.jtcme.2019.05.001.
Durak MA, Parlakpinar H, Polat A, Vardi N, Ekici K, Ucar M, Pasahan R. Protective and therapeutic effects of molsidomine on radiation induced neural injury in rats. Biotechnic & Histochemistry. 2017; 92(1):68–77. https://doi.org/10.1080/10520295.2016.1271454.
Safonov AA. Synthesis, transformation, physicochemical and biological properties of 1, 2, 4-triazole derivatives containing a thiophen-2-ylmethyl substituent (Doctoral dissertation, Zaporizhzhia State Medical University), 2023 [CrossRef].
