CHANGES IN INTER- AND INTRA DYFFERON SKIN TISSUES HETEROMORPHISM UNDER CONDITIONS OF INFLUENCE OF SILVER NANOPARTICLES OF 20, 30, 70 NM
Keywords:
heteromorphism tissue, nanoparticlesAbstract
The study is focused on developing of morphological criteria of biological tissue reactions to metal nanoparticles by detecting changes of tissues heteromorphism interacting with NPs. The study of heteromorphism tissue provides an integrated assessment of functional state of the tissue, allowing objectively evaluate the response of biological tissues in metal nanoparticles. Size-dependent effects of silver nanoparticles were identified, namely depending on the nanoparticles size recovery rate of basement membrane structure differs; the increase of mitotic index of the epidermal basal cells; changes of dermal fibroblasts’s heteromorphism, such as increasing of number of functionally active fibroblasts; and the number of collagen fibers of the dermis. Reactive changes of intradifferon heteromorphism of epidermal basal cells and the dermal fibroblasts was described using quantitative histological methods.
References
Mehlich J. Nanomedicine: visions, risk, potential / J. Mehlich, F. Thiele // European journal of nanomedicine. – 2014. – N 1. – P. 47–53.
Біологічні аспекти наномедицини / І. С. Чекман, В. Ф. Шаторна, О. О. Савенкова [та ін.] // Вісник проблем біології і медицини. – 2011. – № 4. – С. 31–36.
Nano-bio effects: interaction of nanomaterials with cells / L. C. Cheng, X. Jiang, J. Wang [et al.] // Nanoscale. – 2013. – N 9. – P. 3547–3569.
Arora S. Cellular responses induced by silver nanoparticles: in vitro studies / S. Arora, J. Jain, J. Rajwade // Toxicology letters. – 2008. – N 179. – Р. 93–100.
Risk assessment principle for engineered nanotechnology in food and drug / M. Hwang, E. J. Lee, S. Y. Kweon [et al.] // Toxicological results. – 2012. – N 2. – Р. 73–79.
Мішалов В. Д. Про правові, законодавчі та етичні норми і вимоги при виконанні наукових досліджень / В. Д. Мішалов, Ю. Б. Чайковський, І. В. Твердохліб // Морфологія. – 2007 – № 2. – С. 108–115.
Гоцульский В. Я. Коррелометр для случайных импульсных сигналов / В. Я. Гоцульский, В. Е. Чечко, В. Г. Заремба // ПТЭ. – 1997. – № 2. – С. 161–162.
Микроскопическая техника: Руководство / под ред. Д. С. Саркисова, Ю. Л. Перова. – М. : Медицина, 1996. – 544 с.
Автандилов Г. Г. Медицинская морфометрия: рукoводство / Г. Г. Автандилов. – М. : Медицина, 1990. – 283 с.
Kwan, K. H. Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing / K. H. Kwan, X. Liu, K. T. Michael // Nanomedicine: nanotechnology, biology, and medicine. – 2011. – N 7. – P. 497–504.
Lee P. Y. Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing / P. Y. Lee, C. M. Ho, V. Lui / ChemMedChem. – 2010. – N 5. – P. 468–475.
Мусина Л. А. Ультраструктура макрофагов, выявляемых при имплантации аллогенного биоматериала Аллоплант / Л. А. Мусина, С. А. Муслимов, А. И. Лебедева // Морфология. – 2006. – № 1. – С. 53–56.
Size dependent macrophage responses and toxicological effects of Ag nanoparticles / J. Park, D. H. Lim, H. J. Lim [et al.] // Chemical Communications. – 2011. – N 47. – P. 4382– 4384.
Gunasekaran T. Silver nanoparticles as real topical bullets for wound healing / T. Gunasekaran, T. Nigusse, M. D. Dhanaraju // Journal of the American college of clinical wound specialists. – 2012. – N 3. – Р. 82–96.
