MODELING CHRONIC STRESS IN LABORATORY RATS USING SOUNDS OF DIFFERENT RANGES
DOI:
https://doi.org/10.32782/2226-2008-2024-6-1Keywords:
experiment, stress, sounds, rats, biophysicsAbstract
Under conditions of modern urbanized society, constant exposure to stressful situations is becoming a significant etiological factor in the development of many diseases. To study the efficacy of new anti-stress drugs, adequate models of chronic stress in laboratory animals are needed to avoid resistance to the stress factor and provide the natural progression of the adaptation syndrome. The aim of the study was to evaluate the stages of adaptation syndrome in the modeling of sound stress by testing the behavior of laboratory rats in an open field system. Materials and methods. To reproduce the stress, an ultrasonic pest repeller LS-912 “Leaven Enterprise” was used, which was turned on for 6 hours a day according to the following scheme: 2 days – ultrasound with a frequency of 30 kHz, the next 2 days – 40 kHz, the next 2 days – 50 kHz, the next 2 days – 65 kHz. Then the scheme was repeated. Testing in the “open field” was carried out for 3 minutes per rat on the 17th, 23rd, and 46th day of the experiment in terms of the “hole-board test”, grooming, horizontal and vertical locomotor activity. The behavioral activity of stressed rats was compared with the corresponding indicators in intact animals. Comparison of mean values between the intact group and the group of rats exposed to sound stress was performed using Student’s t-test. The results were considered significantly different at p<0.05. Results. It was found that on the 17th day, sound stress caused an increase in animal anxiety with a 2.4-fold increase in the amount of grooming. On the 23rd day of the experiment, an increase in the overall hyperactivity of stressed animals by 46.3% was recorded, indicating their excessive excitement and fear. On the 46th day of observation, rats exposed to sound stress showed a significant decrease in locomotor, cognitive activity and general behavioral actions by almost 2 times, which corresponds to the stage of exhaustion. The obtained results allow the use of the LS-912 pest repeller with a change in the ultrasound range every 2–3 days to model chronic stress in laboratory rats in order to study the effectiveness of new anti-stress drugs.
References
Hill RJ, McKernan LC, Wang L, Coronado RA. Changes in psychosocial well-being after mindfulness-based stress reduction: a prospective cohort study. J Man Manip Ther. 2017; 25(3): 128–136. doi: 10.1080/10669817.2017.1323608.
Wang C, Wang XR, Song DD, et al. The establishment of a rat model in myocardial ischemia with psychological stress. Ann Transl Med. 2020; 8(6): 322. doi: 10.21037/atm.2020.02.128.
Plotnikova LM, Berezovskyi VY, Levashov MI, Chaka OG, Yanko RV. Vplyv hipoksiyi ta melatoninu na povedinkovu reaktsiyu shchuriv z riznym rivnem energetychnoho metabolizmu [The influence of hypoxia and melatonin on the behavioral response of rats with different levels of energy metabolism]. Fiziologichnyi Zhurnal. 2017; 63(6): 66–71. (In Ukrainian). Available from: https://fz.kiev.ua/journals/2017_V.63/2017_6/6-66-71.pdf.
Pimple P, Lima BB, Hammadah M, et al. Psychological Distress and Subsequent Cardiovascular Events in Individuals With Coronary Artery Disease. J Am Heart Assoc. 2019; 8(9): e011866. doi: 10.1161/JAHA.118.
Pshychenko VV, Cherno VS. Vplyv khronichnoho stresu na morfometrychni pokaznyky pinealnykh klityn shchuriv [Effect of chronic stress on morphometric parameters of rat pineal cells]. Visnyk problem biolohii ta medytsyny. 2018; 2(147): 298–300. (In Ukrainian). doi: 10.29254/2077-4214-2018- 4-2-147-298-300.
Savotchenko AV, Semenikhina MO, Krasnianchuk IV, Bogovyk RI, Honcharova AE, Isaeva EV. Behavioral reaktsiyi shchuriv pry enterobiozi [Behavioral reactions of rats with enterobiosis]. Fiziologichnyi Zhurnal. 2019; 65(1): 20–25. (In Ukrainian). Available from: https://fz.kiev.ua/journals/2019_V.65/2019-1/2019-1-20-25.pdf.
Bilets MV, Omelchenko OY, Vesnina LE, Mamontova TV, inventors; Ukrainian Medical Dental Academy, assignee. Sposib modelyuvannya pidvyshchenoyi chutlyvosti shchuriv do khronichnoho stresu [A method for modeling the increased sensitivity of rats to chronic stress]. Ukrainian patent 127604. 2018 March 19.
Miskiv VA, Zhurakivska OY, Paliichuk IV, et al., inventors; Ivano- Frankivsk National Medical University, assignee. Sposib modelyuvannya khronichnoho stresu u eksperymental’nykh tvaryn riznykh vikovykh hrup [Method for modeling chronic stress in experimental animals of different age groups]. Ukrainian patent 125623. 2022 May 4.
Hrabovska SV. Neyrofiziolohichni reaktsiyi na diyu nyz’kykh doz khlorpiryfosu u shchuriv i yikh potomstva [Neurophysiological reactions to low doses of chlorpyrifos in rats and their offspring]. Dissertation of Candidate of Biological Sciences (Doctor of Philosophy). Lviv: Ivan Franko National University of Lviv; 2018. 132 p. https://uacademic.info/ua/document/0418U005423#!
Sturman O, Germain PL, Bohacek J. Exploratory rearing: a context- and stress-sensitive behavior recorded in the open-field test. Stress. 2018; 21(5): 443–452. doi: 10.1080/10253890.2018.1438405.
Hrabovska SV, Salyha YT. Vplyv khronichnoyi intoksykatsiyi nyz’kymy dozamy khlorpiryfosu na povedinku samtsiv shchuriv [The effect of chronic intoxication with low doses of chlorpyrifos on the behavioral parameters of female rats]. Fiziologichnyi Zhurnal. 2015; 61(2): 94–101. (In Ukrainian). doi: 10.15407/fz61.02.094.
Kozlova YV. Behavior of Rats in the Open Field within the Early Period after Light-Degree Blast-Induced Neurotrauma. Neurophysiology. 2021; 53(2): 101–108. https://doi.org/10.1007/s11062-022-09921-z.
Zinovieva ML, Karpezo NO, Lynchak OV. Osoblyvosti povedinkovykh reaktsiy samok i samtsiv shchuriv za subkhronichnoyi diyi 7- hidroksykumarynu [Peculiarity of the Behavioral Reactions of Rats Males and Females Exposed Subchronically to 7-Hydroxicoumarin]. Ukrainian journal suchasnykh problem toksykolohii. 2014; 5: 45–49. (In Ukrainian). Available from: http://protox.medved.kiev.ua/index.php/ua/issues/2014/5/item/431-peculiarity-of-the-behavior-reactions-of-rats-malesand-females-exposed-subchronically-to-7-hydroxicoumarin.
Antipova RV, Komisova TE, Sak AY. Osoblyvosti povedinkovykh reaktsiy samtsiv shchuriv u testi “vidkryte pole” pry alimintarnomu nadkhodzhenni zhyriv riznoho pokhodzhennya [Peculiarities of behavioral reactions of male rats in the open field test in case of alimentary intake of fats of different origins]. Bioriznomanittya, ekolohiya ta eksperymental’na biolohiya. 2020; 22(1): 9–20 (In Ukrainian). https://doi.org/10.34142/2708-5848.2020.22.1.01.
