NEUTROPHIL-TO-LYMPHOCYTE AND LYMPHOCYTE-TO-MONOCYTE RATIO IN THE PERIPHERAL BLOOD OF RATS WITH SECONDARY CHRONIC CARRAGEENAN INFLAMMATION AFTER ADMINISTRATION OF THE THROMBIN BLOCKER DABIGATRAN ETEXILATE
DOI:
https://doi.org/10.32782/2226-2008-2023-4-2Keywords:
secondary chronic carrageenan inflammation, neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio, peripheral blood, dabigatran etexilateAbstract
Introduction. Neutrophil-lymphocyte and lymphocyte-monocyte ratios determine the activity of the inflammatory process and are widely used as prognostic criteria for the development of various diseases and the rate of wound healing. The lymphocyte-monocyte ratio, along with the levels of proinflammatory cytokines in peripheral blood, can be reliably used to predict the course of chronic inflammation. Objective. To determine neutrophil-lymphocyte and lymphocyte-monocyte ratios in the peripheral blood of rats with secondary chronic carrageenan inflammation after administration of the thrombin blocker – dabigatran etexilate. Materials and methods. The experimental study was conducted on 72 adult male WAG rats weighing 180–200 g. The model of inflammation was a secondary chronic aseptic inflammation induced by intramuscular injection of 10 mg of λ-carrageenan (Sigma, USA) into the thigh area. Dabigatran etexilate was administered intragastrically through a gavage tube at a dose of 15 mg/kg/day daily throughout the experiment. The neutrophil-lymphocyte and lymphocyte-monocyte ratio in the peripheral blood was studied on days 1, 7, 14, 21, and 28 of the experiment. Results. When comparing the neutrophil-lymphocyte ratio in secondary chronic carrageenan inflammation after administration of dabigatran etexilate with that in the natural course of inflammation, a significant increase in it was observed on day 14 by 3.1 times, p<0,05, and the lymphocyte-monocyte ratio significantly increased on day 28 by 1.3 times, p<0,01. Conclusion. The data obtained indicates a positive dynamic of the course of secondary chronic carrageenan inflammation after administration of dabigatran etexilate in comparison with its natural course.
References
Libby P. Inflammatory mechanisms: the molecular basis of inflammation and disease. Nutr Rev. 2007;65(12Pt2):140-146. doi: 10.1111/j.1753-4887.2007.tb00352.x. PMID: 18240538.
Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007; 147(2): 227-235. doi: 10.1111/j.1365-2249.2006.03261.x. PMID: 17223962; PMCID: PMC1810472.
Song M, Graubard BI, Rabkin CS, Engels EA. Neutrophil-to-lymphocyte ratio and mortality in the United States general population. Sci Rep. 2021; 11(1): 464. doi: 10.1038/s41598-020-79431-7. PMID: 33431958; PMCID: PMC7801737.
Mortaz E, Alipoor SD, Adcock IM, Mumby S, Koenderman L. Update on Neutrophil Function in Severe Inflammation. Front Immunol. 2018;9:2171. doi: 10.3389/fimmu.2018.02171. PMID: 30356867; PMCID: PMC6190891.
Lowsby R, Gomes C, Jarman I, et al. Neutrophil to lymphocyte count ratio as an early indicator of blood stream infection in the emergency department. Emerg Med J. 2015; 32(7): 531-534. doi: 10.1136/emermed-2014-204071. Epub 2014 Sep 2. PMID: 25183249.
Lee JS, Kim NY, Na SH, Youn YH, Shin CS. Reference values of neutrophil-lymphocyte ratio, lymphocyte-monocyte ratio, platelet-lymphocyte ratio, and mean platelet volume in healthy adults in South Korea. Medicine (Baltimore). 2018; 97(26): e11138. doi: 10.1097/MD.0000000000011138. PMID: 29952958; PMCID: PMC6039688.
Sierzega M, Lenart M, Rutkowska M, et al. Preoperative Neutrophil-Lymphocyte and Lymphocyte-Monocyte Ratios Reflect Immune Cell Population Rearrangement in Resectable Pancreatic Cancer. Ann Surg Oncol. 2017; 24(3): 808-815. doi: 10.1245/s10434-016-5634-0. Epub 2016 Oct 21. PMID: 27770341; PMCID: PMC5306070.
Shibutani M, Maeda K, Nagahara H, Iseki Y, Ikeya T, Hirakawa K. Prognostic significance of the preoperative lymphocyteto-monocyte ratio in patients with colorectal cancer. Oncol Lett. 2017;13(2):1000-1006. doi: 10.3892/ol.2016.5487. Epub 2016 Dec 12. PMID: 28356991; PMCID: PMC5351160.
Radhakrishnan R, Moore SA, Sluka KA. Unilateral carrageenan injection into muscle or joint induces chronic bilateral hyperalgesia in rats. Pain. 2003;104(3): 567-577. doi: 10.1016/S0304-3959(03)00114-3. PMID: 12927629; PMCID: PMC2732018.
Dittmeier M, Wassmuth K, Schuhmann MK, Kraft P, Kleinschnitz C, Fluri F. Dabigatran Etexilate Reduces Thrombin-Induced Inflammation and Thrombus Formation in Experimental Ischemic Stroke. Curr Neurovasc Res. 2016;13(3):199-206. doi: 10.2174/1567202613666160517122605. PMID: 27184031.
Durmaz S, Kurtoğlu T, Rahman ÖF, et al. Direct oral anticoagulant agents attenuate temporary aortic occlusion-induced renal oxidative and inflammatory responses in rats. Turk Gogus Kalp Damar Cerrahisi Derg. 2022; 30(2): 184-191. doi: 10.5606/tgkdc.dergisi.2022.22831. PMID: 36168569; PMCID: PMC9473587.
Vogler GA. Anesthesia and Analgesia. In: Suckow MA, Weisbroth SH, Franklin CL, editors. The Laboratory Rat. 2nd ed. Burlington (MA): Academic Press; 2006. Chapter 19. p. 627-664. doi: 10.1016/B978-012074903-4/50022-4.
Abdi-Azar H, Abbasi-Maleki S. Comparison of the anesthesia with thiopental sodium alone and their combination with Citrus aurantium L. (Rutaseae) essential oil in male rat. Bull Env Pharmacol Life Sci. 2014; 3: 37-44.
Mishra P, Singh U, Pandey CM, Mishra P, Pandey G. Application of student's t-test, analysis of variance, and covariance. Ann Card Anaesth. 2019; 22(4): 407-411. doi: 10.4103/aca.ACA_94_19. PMID: 31621677; PMCID: PMC6813708.
