EXPERIMENTAL EVALUATION OF BIOCHEMICAL MARKERS OF RAT ORAL MUCOSA AGAINST THE BACKGROUND OF MODELING PEROXIDE PERIODONTITIS AND TREATMENT-PREVENTION MEASURES
DOI:
https://doi.org/10.32782/2226-2008-2024-1-1Keywords:
peroxidized periodontitis, bone tissue, rats, experiment, biochemical markersAbstract
A deep understanding of the biochemical changes occurring in the periodontium during the development and progression of periodontitis is important for developing effective methods of treatment and prevention of this disease. The aim of the present study was to experimentally evaluate biochemical markers of the oral mucosa of rats against the background of modeling peroxidative periodontitis and treatment-prevention measures. Materials and methods. During the experiment, changes in biochemical parameters of oral mucosa under the influence of therapeutic and prophylactic measures developed for patients with varying degrees of periodontal tissue damage were evaluated using a model of peroxidative periodontitis. The experiment involved 32 two-month-old Wistar rats of herd breeding. The rats were divided into groups of 8 rats each. A statistically significant difference between alternative quantitative features with a distribution corresponding to the normal law was evaluated using Student’s t-test. The difference was considered statistically significant at p<0.01. Results. The experiment determined that the presence of inflammation and a high level of contamination with opportunistic bacteria was recorded in the oral mucosa of rats with peroxidative periodontitis. Additional administration of the treatment and preventive complex (TPC) to rats with periodontitis contributed to a more pronounced periodontal protective effect: in the oral mucosa of rats under the influence of complex, markers of inflammation, microbial contamination, lipid peroxidation and antioxidant system activity did not differ from the corresponding level in healthy rats. In addition, complex showed a pronounced hepatoprotective effect in animals with prolonged use of peroxidized oil.
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