THE RELATIONSHIP BETWEEN THE EXPRESSION OF MICRORNA-29B AND -146A WITH CLINICAL AND PATHOLOGICAL CHARACTERISTICS OF PATIENTS WITH UTERINE FIBROIDS
DOI:
https://doi.org/10.32782/2226-2008-2023-2-4Keywords:
uterine fibroids, epigenetics, biomarkers of prognosis, miRNA, diagnosticsAbstract
In order to investigate the relationship between the expression of miRNA-29b and -146a and the clinical and pathological characteristics of patients with uterine myoma, 28 patients with uterine myoma were examined, in whom the expression of miRNA in tumor tissue was determined using polymerase chain reaction (PCR) in real time . Analysis of miRNA-29b and -146a expression levels in the tumor tissue of patients with uterine fibroids, establishing their significant variability. The average expression level of microRNA-29b was 4.6±0.5 units, miRNA-146a – 52.0±4.8 units. A direct correlation between body mass index (BMI) and miRNA-29b and miRNA-146a expression was established (p<0.05), as well as miRNA-29b expression and uterine vessel dopplerometry indicators (p<0.05). However, an inverse correlation of miRNA-146a expression with the resistance index (p<0.05) and the pulsatility index of the node (p<0.05) was found in the myomatous node tissue.
The article is available at the Institutional Repository of Odesa National Medical University http://journal.odmu.edu.ua/?p=7571&lang=en
References
Harris HR, Petrick JL, Rosenberg L. The epidemiology of uterine fibroids: Where do we go from here? Fertil Steril. 2022 Apr;117(4):841-842. doi: 10.1016/j.fertnstert.2022.01.037. Epub 2022 Mar 9. PMID: 35277259.
Yang Q, Ciebiera M, Bariani MV, Ali M, Elkafas H, Boyer TG, Al-Hendy A. Comprehensive Review of Uterine Fibroids: Developmental Origin, Pathogenesis, and Treatment. Endocr Rev. 2022 Jul 13; 43(4):678-719. doi: 10.1210/endrev/bnab039.Erratum in: Endocr Rev. 2022 Mar 02;: Erratum in: Endocr Rev. 2022 Mar 02;: PMID: 34741454; PMCID: PMC9277653.
Stewart EA, Nowak RA. Uterine Fibroids: Hiding in Plain Sight. Physiology (Bethesda). 2022 Jan 1; 37(1):16-27. doi: 10.1152/physiol.00013.2021. PMID: 34964688; PMCID: PMC8742728.
Baranov VS, Osinovskaya NS, Yarmolinskaya MI. Pathogenomics of Uterine Fibroids Development. Int J Mol Sci. 2019 Dec 6; 20(24):6151. doi: 10.3390/ijms20246151. PMID: 31817606; PMCID: PMC6940759.
Zota AR, Geller RJ, VanNoy BN, Marfori CQ, Tabbara S, Hu LY, Baccarelli AA, Moawad GN. Phthalate Exposures and MicroRNA Expression in Uterine Fibroids: The FORGE Study. Epigenet Insights. 2020 Feb 18;13:2516865720904057. doi: 10.1177/2516865720904057. PMID: 32128507; PMCID: PMC7031793.
Islam MS, Ciavattini A, Petraglia F, Castellucci M, Ciarmela P. Extracellular matrix in uterine leiomyoma pathogenesis: a potential target for future therapeutics. Hum Reprod Update. 2018 Jan 1;24(1):59-85. doi: 10.1093/humupd/dmx032. PMID: 29186429.
Ciebiera M, Włodarczyk M, Zgliczyński S, Łoziński T, Walczak K, Czekierdowski A. The Role of miRNA and Related Pathways in Pathophysiology of Uterine Fibroids-From Bench to Bedside. Int J Mol Sci. 2020 Apr 24; 21(8):3016. doi: 10.3390/ijms21083016. PMID: 32344726; PMCID: PMC7216240.
ACOG guideline. Management of symptomatic uterine leiomyomas. Retrieved from: https://www.acog.org/clinical/clinicalguidance/practice-bulletin/articles/2021/06/management-of-symptomatic-uterine-leiomyomas.
LABOME MicroRNA Experimental Protocols. Retrieved from: https://www.labome.com/method/MicroRNA-Experimental-Protocols.html#:~:text=Basic%20principle%3A%20microRNA%20RT%2DPCR,transcription%20of%20small%20RNA%20U6.
Genomics.Hu Project. Retrieved from: http://genomics.hu.
Torres A, Torres K, Wdowiak P, Paszkowski T, Maciejewski R. Selection and validation of endogenous controls for microRNA expression studies in endometrioid endometrial cancer tissues. Gynecol Oncol. 2013 Sep;130(3):588-94. doi: 10.1016/j.ygyno.2013.06.026. Epub 2013 Jun 26. PMID: 23811003.
Wang, T., Zhang, X., Obijuru, L., Laser, J., Aris, V., Lee, P., ... & Wei, J. J. (2007). A micro‐RNA signature associated with race, tumor size, and target gene activity in human uterine leiomyomas. Genes, Chromosomes and Cancer, 46(4), 336–347.
Chuang, T. D., & Khorram, O. (2019). Regulation of cell cycle regulatory proteins by microRNAs in uterine leiomyoma. Reproductive Sciences, 26(2), 250–258.
Xu, X., Kim, J. J., Li, Y., Xie, J., Shao, C., & Wei, J. J. (2018). Oxidative stress-induced miRNAs modulate AKT signaling and promote cellular senescence in uterine leiomyoma. Journal of Molecular Medicine, 96(10), 1095–1106.
Qiang, W., Liu, Z., Serna, V. A., Druschitz, S. A., Liu, Y., Espona-Fiedler, M., ... & Kurita, T. (2014). Down-regulation of miR-29b is essential for pathogenesis of uterine leiomyoma. Endocrinology, 155(3), 663–669.
