ANALGESIC POTENTIAL OF CRYOEXTRACTS OF BIOLOGICAL TISSUES AND CONDITIONED MEDIA OF MESENCHYMAL STEM CELLS IN THE TREATMENT OF EXPERIMENTAL AUTOIMMUNE ARTHRITIS
DOI:
https://doi.org/10.32782/2226-2008-2024-1-6Keywords:
cell-free cryopreserved biological agents, autoimmune diseases, spleen cryoextract, placenta cryoextract, mesenchymal stem cell conditioned mediumAbstract
Background. Treating pain syndrome remains the focus of clinicians’ attention, and developing new treatment methods is an urgent task of modern medicine. Particular attention is drawn to the results of recent studies on the use of cell-free cryopreserved biological agents to optimize anti-inflammatory and analgesic therapy for a number of autoimmune diseases. Objective – to characterize the analgesic activity of cryoextract of placenta (CEP), cryoextract of spleen, and conditioned medium of mesenchymal stem cells in the model of autoimmune arthritis (АА) in rats. Methods. Experimental studies were conducted on 42 male rats weighing 200–220 g. AA was modeled by sub-plantar administration of complete Freund’s adjuvant. AA treatment was carried out from 14 to 28 days. On “0” (initial indicators), 14 and 28 days of the experiment, pain sensitivity was assessed (electrical impulse irritation and mechanical irritation according to Randall-Selitto). Results. The conducted study showed that on the 14th day of the experiment in rats with simulated AA, a statistically significant (p<0.009) decrease by 2 times in the threshold of pain sensitivity with mechanical irritation according to Randall-Selitto was observed relative to the initial indicators. Evaluation of the effect of administration of biological agents to rats with AA demonstrated the ability of the studied drugs to reduce the threshold of pain sensitivity from the action of a mechanical stimulus, which indicates the modulation of the peripheral component of nociception. Against the background of the introduction of СEP to rats with AA, a statistically significant (p=0.01) increase in threshold of pain sensitivity from an electrical impulse stimulus was noted by 44.7%. Conclusions. At the spinal level of nociception, cryoextract of placenta and conditioned medium of mesenchymal stem cells showed comparable analgesic activity, which was 43.1% and 42.7%, respectively. At the supraspinal level of nociception, the most pronounced analgesic activity was found in the conditioned medium of mesenchymal stem cells, which was 54.8%, that is only 18.8% lower (p=0.049) than the indicators of rats administered diclofenac sodium.
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