CHARACTERISTICS OF INDIVIDUAL COMPONENTS OF THE HUMORAL AND CELLULAR LINKS OF IMMUNITY IN THE BLOOD OF GUINEA PIGS WITH EXPERIMENTAL PERIODONTITIS

Authors

DOI:

https://doi.org/10.32782/2226-2008-2023-4-3

Keywords:

experimental periodontitis, T-lymphocytes, B-lymphocytes, circulating immune complexes

Abstract

The pathogenesis of periodontitis mainly involves the disease-associated oral microbiota, inflammation of the body and environmental and genetic risk factors. It has been established that the immune reactions of the human immune system determine susceptibility to periodontal disease. However, the precise role of different immune cells in periodontitis, the role of immunity in alveolar bone destruction, and the specific signaling pathways involved in immune regulation in periodontitis remain obscure. The aim of the study is to establish the state of individual components of the humoral and cellular links of immunity in the guinea pigs’ blood with experimental periodontitis. Research materials and methods. Experimental studies were conducted on 46 guinea pigs (males) weighing 180–220 g, divided into 5 groups of 9 animals in each except for the first (10 animals). The model of experimental periodontitis was reproduced following the method of O. N. Voskresensky, determination of the content of T- and B-lymphocytes in the blood – following the method of Chernushenko E. F., Kogosova L. S., determination of circulating immune complexes in blood – following the method of V. V. Menshikova. Statistical processing of the obtained data was carried out through the Student’s t-test. The obtained results showed a significant decrease in the number of T-lymphocytes for all days of the experimental simulation of the disease against the background of an increase in the content of B-lymphocytes and circulating immune complexes which indicates the depression of the cellular mechanisms of the body’s defense and the activation of the humoral link of the immune response during the development of experimental periodontitis.

References

He W, You M, Wan W, Xu F, Li F, Li A. Point-Of-Care Periodontitis Testing: Biomarkers, Current Technologies, and Perspectives. Trends Biotechnol. 2018; 36 (11): 1127–1144. https://doi.org/10.1016/j.tibtech.2018.05.013.

Hajishengallis G, Chavakis T, Lambris JD. Current Understanding of Periodontal Disease Pathogenesis and Targets for Host-Modulation Therapy. Periodontol 2000. 2020; 84 (1): 14–34. DOI: 10.1111/prd.12331.

Teles F, Wang Y, Hajishengallis G, Hasturk H, Marchesan JT. Impact of Systemic Factors in Shaping the Periodontal Microbiome. Periodontol 2000. 2021; 85 (1): 126–160. DOI: 10.1111/prd.12356.

Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal Diseases. Nat Rev Dis Primers. 2017; 3: 138–170. DOI: 10.1038/nrdp.2017.38.

Zhu M, Belkina AC, DeFuria J et al. B cells promote obesity-associated periodontitis and oral pathogen-associated inflammation. J Leukoc Biol. 2014; 96: 34357. DOI: 10.1189/jlb.4A0214-095R.

Oliver-Bell J, Butcher JP, Malcolm J et al. Periodontitis in the absence of B cells and specific anti-bacterial antibody. Mol Oral Microbiol. 2014; 30 (1): 160–169. DOI: 10.1111/omi.12082.

Park H, Li Z, Yang XO et al. A Distinct Lineage of CD4 T Cells Regulates Tissue Inflammation by Producing Interleukin 17. Nat Immunol. 2005; 6 (11): 1133–1141. DOI: 10.1038/ni1261.

Bunte K, Beikler T. Th17 Cells and the IL-23/IL-17 Axis in the Pathogenesis of Periodontitis and Immune-Mediated Inflammatory Diseases. Int J Mol Sci. 2019; 20 (14): 339–346. DOI: 10.3390/ijms20143394.

Wilson NJ, Boniface K, Chan JR et al. Development, Cytokine Profile and Function of Human Interleukin 17-Producing Helper T Cells. Nat Immunol. 2007; 8 (9): 950–957. DOI: 10.1038/ni1497.

Dutzan N, Abusleme L. eds. T Helper 17 Cells as Pathogenic Drivers of Periodontitis. Cham: Springer International Publishing; 2019. DOI: 10.3389/fimmu.2021.742925.

Kuramoto A, Yoshinaga Y, Kaneko T et al. The formation of immune complexes is involved in the acute phase of periodontal destruction in rats. J Periodontal Res. 2012; (47): 455–462. DOI:10.3389/fimmu.2021.591236.

Marques CPC, Maor Y, De Andrade MS, Rodrigues VP, Benatti BB. Possible evidence of systemic lupus erythematosus and periodontal disease association mediated by toll-like receptors 2 and 4. Clin Exp Immunol. 2016; 183: 187–192. DOI: 10.1111/cei.12708.

Voskresensky ON. Preclinical study of means of prevention and treatment of periodontitis (periodontoprotectors). Guidelines. К.: Аvicenna. 2002; 16 (in Ukrainian).

Chernyshenko IF, Kogosova LS. Immunology and immunopathology of pulmonary diseases. Guidelines. К.: Zdorovja.1981; 208 (in Ukrainian).

Menshukov VV. Laboratory methods of clinical investigations. Guidelines. Меdicina 1987; 292 (in Ukrainian).

Published

2023-12-28

Issue

Section

THEORY AND EXPERIMENT